====== About ======

**PolyPhen-2** is a new development of the **PolyPhen** tool for annotating coding nonsynonymous SNPs. Some of the highlights of the new version are:

  * High quality multiple sequence alignment pipeline
  * Probabilistic classifier based on machine-learning method
  * Optimized for high-throughput analysis of the next-generation sequencing data

== Please cite: ==

  * Adzhubei IA, Schmidt S, Peshkin L, Ramensky VE, Gerasimova A, Bork P, Kondrashov AS, Sunyaev SR. Nat Methods **7**(4):248-249 (2010). [[http://www.ncbi.nlm.nih.gov/pubmed/20354512|PubMed]] {{nmeth0410-248.pdf|PDF}} {{nmeth0410-248-s1.pdf|Supplemental Information}}

== Other References: ==

  - Adzhubei I, Jordan DM, Sunyaev SR. Predicting functional effect of human missense mutations using PolyPhen-2. Curr Protoc Hum Genet, Chapter 7:Unit7.20 (2013). [[http://www.ncbi.nlm.nih.gov/pubmed/23315928|PubMed]] {{hg0720.pdf|PDF}}
  - Ramensky V, Bork P, Sunyaev S. Human non-synonymous SNPs: server and survey. Nucleic Acids Res **30**(17):3894-3900 (2002). [[http://www.ncbi.nlm.nih.gov/pubmed/12202775|PubMed]] {{nar-2002-3894.pdf|PDF}}
  - Sunyaev SR, Eisenhaber F, Rodchenkov IV, Eisenhaber B, Tumanyan VG, Kuznetsov EN. PSIC: profile extraction from sequence alignments with position-specific counts of independent observations. Protein Eng **12**(5):387-394 (1999). [[http://www.ncbi.nlm.nih.gov/pubmed/10360979|PubMed]] {{peds-1999-387.pdf|PDF}}